Ayuno

Ayuno is the femslash ship between Aya Asagiri and Tsuyuno Yatsumura from the Mahou Shoujo Site fandom.

Canon
Both Aya and Tsuyuno are introduced during Chapter 1 of the manga and Episode 1 of the anime.

Tsuyuno is Aya's upperclassman, who saves her from having her mouth cut open by Sarina in the girls' bathroom.

Their relationship started off as a partnership, being mutually-beneficial for both of them. Over time, they became closer and eventually thought of each other as close friends, but, as the series goes on it's shown their feelings get much deeper than that as at the end of chapter 54, Aya and Tsuyuno share a kiss which implies their attraction to one another was romantic.

Chapter 86 of the manga reveals that Tsuyuno's death traumatised Aya to the point she became mute. Which further proves how important Tsuyuno and Aya were to each other.

Fanon
Both Aya and Tsuyuno could potentially be LGBT, as most fans theorise. Aya and Tsuyuno both are shown to be attracted to each other, meaning people of the same gender.

This wouldn't be shocking as Mahou Shoujo Site has a canonically transgendered character.

Fandom
FAN FICTION
 * Aya/Tsuyuno tag on Archive of Our Own.

Trivia

 * The anime adaptation of Mahou Shoujo Site strays from the timeline of the manga, it also removes the kiss scene that happens in chapter 54.